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mouse anti synaptotagmin  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mouse anti synaptotagmin
    Mouse Anti Synaptotagmin, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+synaptotagmin/anti-Synaptotagmin/pm41760544-207-149-155
    Average 94 stars, based on 17 article reviews
    mouse anti synaptotagmin - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Parallel evolution of a splicing program controlling neuronal excitability in flies and mammals
    Article Snippet: Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI), captured with a Leica SP5 confocal microscope, and processed with Fiji ( ). .. For NMJ staining, third-instar larvae were dissected in cold PBS and fixed with 4% paraformaldehyde in PBS for 45 min. Larvae were then washed in PBS-T (PBS + 0.5% Triton X-100) six times for 30 min and incubated overnight at 4°C with mouse anti-synaptotagmin, 1:200 (3H2 2D7, Developmental Studies Hybridoma Bank). .. After six 30-min washes with PBS-T, secondary antibody anti-mouse conjugated to Alexa Fluor 488 and tetramethyl rhodamine isothiocyanate–conjugated anti-HRP (Jackson ImmunoResearch) were used at a concentration of 1:1000 and incubated at room temperature for 2 hours.

    Article Title: The ribose methylation enzyme FTSJ1 has a conserved role in neuron morphology and learning performance
    Article Snippet: .. For NMJ staining, third instar larvae were dissected in cold PBS and fixed with 4% paraformaldehyde in PBS for 45 min. Larvae were then washed in PBST (PBS + 0.5% Triton X-100) six times for 30 min and incubated overnight at 4°C with mouse anti-synaptotagmin, 1:200 (3H2 2D7, Developmental Studies Hybridoma Bank, DSHB). .. After six 30-min washes with PBST, secondary antibody anti-mouse conjugated to Alexa Fluor 488 and TRITC-conjugated anti-HRP (Jackson ImmunoResearch) were used at a concentration of 1:1,000 and incubated at room temperature for 2 h. Larvae were washed again six times with PBST and finally mounted in Vectashield (Vector Laboratories).

    Incubation:

    Article Title: Parallel evolution of a splicing program controlling neuronal excitability in flies and mammals
    Article Snippet: Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI), captured with a Leica SP5 confocal microscope, and processed with Fiji ( ). .. For NMJ staining, third-instar larvae were dissected in cold PBS and fixed with 4% paraformaldehyde in PBS for 45 min. Larvae were then washed in PBS-T (PBS + 0.5% Triton X-100) six times for 30 min and incubated overnight at 4°C with mouse anti-synaptotagmin, 1:200 (3H2 2D7, Developmental Studies Hybridoma Bank). .. After six 30-min washes with PBS-T, secondary antibody anti-mouse conjugated to Alexa Fluor 488 and tetramethyl rhodamine isothiocyanate–conjugated anti-HRP (Jackson ImmunoResearch) were used at a concentration of 1:1000 and incubated at room temperature for 2 hours.

    Article Title: The ribose methylation enzyme FTSJ1 has a conserved role in neuron morphology and learning performance
    Article Snippet: .. For NMJ staining, third instar larvae were dissected in cold PBS and fixed with 4% paraformaldehyde in PBS for 45 min. Larvae were then washed in PBST (PBS + 0.5% Triton X-100) six times for 30 min and incubated overnight at 4°C with mouse anti-synaptotagmin, 1:200 (3H2 2D7, Developmental Studies Hybridoma Bank, DSHB). .. After six 30-min washes with PBST, secondary antibody anti-mouse conjugated to Alexa Fluor 488 and TRITC-conjugated anti-HRP (Jackson ImmunoResearch) were used at a concentration of 1:1,000 and incubated at room temperature for 2 h. Larvae were washed again six times with PBST and finally mounted in Vectashield (Vector Laboratories).



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